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University of Delaware
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| AUTHOR: |
Robin Morgan, Amy Anderson, Erin Bernberg, Sachin
Kamboj, Emily Huang, Grace Lagasse, Grace Isaacs, Mark Parcells, Blake C. Meyers, Pamela J. Green, and
Joan Burnside
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| TITLE: |
Sequence Conservation and Differential Expression of Marek's Disease Virus MicroRNAs
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| ABSTRACT: |
Marek's disease virus (MDV), a herpesvirus that causes
a lymphoproliferative disorder in chickens, encodes
a number of microRNAs derived primarily from two
locations in the MDV genome. One cluster of microRNA
genes flanks the meq oncogene, and a second cluster
is found within the latency-associated transcript
(LAT) region. The sequences of MDV microRNAs from a
collection of field and reference strains with
various levels of virulence were compared and found
to be highly conserved. However, microRNAs from the
meq cluster were detected at higher levels in
lymphomas caused by a form of the virus designated
very virulent plus (vv+; strain 615K, also known as
T. King) than in those caused by a less virulent
(very virulent [vv]) form (RB1B). For example, levels of mdv1-miR-M4, which shares a seed sequence
with miR-155, a microRNA implicated in B-cell
lymphoma, were threefold higher and levels of
mdv1-miR-M2*/3p were more than sixfold higher in vv+
MDV-induced tumors than in vv MDV-induced tumors. In
contrast, levels of the microRNAs from the LAT
cluster were equivalent in tumors produced by vv and
vv+ strains. Additionally, mdv1-miR-M4 is the MDV
microRNA most highly expressed in tumors, where it
accounts for 72\% of all MDV microRNAs, as
determined by deep sequencing. These data suggest
that the meq cluster microRNAs play an important
role in the pathogenicity of MDV.
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| PUBLICATION: |
Journal of Virology
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| DATE: |
2008
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| SOURCES: |
PDF: http://jvi.asm.org/cgi/reprint/82/24/12213
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| DOI: |
http://dx.doi.org/10.1128/JVI.01722-08
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| URL: |
http://jvi.asm.org/cgi/content/abstract/82/24/12213
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| PAGES: |
12213-12220
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| BIBTEX: |
@article{kamboj.jvi.08, Doi = {http://dx.doi.org/10.1128/JVI.01722-08}, Title = {Sequence Conservation and Differential Expression of Marek's Disease Virus MicroRNAs}, Url = {http://jvi.asm.org/cgi/content/abstract/82/24/12213}, Author = {Robin Morgan, Amy Anderson, Erin Bernberg, Sachin Kamboj, Emily Huang, Grace Lagasse, Grace Isaacs, Mark Parcells, Blake C. Meyers, Pamela J. Green, and Joan Burnside}, Source = {http://jvi.asm.org/cgi/reprint/82/24/12213}, Year = {2008}, Pages = {12213-12220}, Journal = {Journal of Virology}, } |
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