Tutorial on Basic DNA Lab Technology for DNA Computing

Fourth International Meeting on
DNA BASED COMPUTING

Junghuei Chen
University of Delaware

Structure and physiological properties of DNA
- DNA can be denatured and renatured
- Singled-Stranded DNA usually has secondary structure
- Duplex DNA has alternative double-helical structures;B, A and Z-forms
- Closed DNA can be supercoiled
Important enzymes relevant to DNA Computing
- Restriction Endonuclease
- DNA Ligase
- DNA Polymerase
Essential Laboratory techniques relevant to DNA Computing
- Gel Electrophoresis separates DNA by mass vs (mostly) drag
- DNA Sequencing Techniques
- PCR (Polymerase Chain Reaction)
- DNA Cloning

This tutorial is from 3pm-5pm June 15, 1998. Registration is free. Preregistration is best, but you may register when you arrive.

Tutorials are held at the DNA 4 Meeting location: Dunlop Auditorium of Stemmler Hall at 3450 Hamilton Walk at the University of Pennsylvania, Philadelphia PA. Enter via Hamilton Walk off of 36th Street Walk. For more detail, including links to maps and transportation, see Local Information.

The other tutorial on June 15 (1pm-3pm) is Tutorial on Formal Language Theory

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Tutorial on Basic DNA Lab Technology for DNA Computing
Compiled by / David Harlan Wood, wood@cis.udel.edu / last revised June 11, 1998